Activation of Myosin Adenosine Triphosphatase by Carbonyl Cyanide P-chlorophenylhydrazone.

In an accompanying paper (l), it is shown that y-(p-arsenosophenyl)-n-butyrate activates the adenosine triphosphatase of both myosin and mitochondria, and several analogies between the properties of the active sites of these two energy transduction enzymes are discussed. Carbonyl cyanide p-chlorophenylhydrazone, which uncouples osidative phosphorylation at concentrations as low as lop7 &r (a), also activates myosin ATPase activity, although, like 2,4-dinitrophenol, the concentration required bo affect the activity of myosin is about loo-fold higher than the concentration required to affect mitochondria. In this paper we present data concerning the interaction between myosin and carbonyl cyanide p-chlorophenylhydrazone, and the effect of certain thiol compounds on this interaction.